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Morning sessions: 8:30a-12:45p (except Tuesday morning starts at 8:00); afternoon sessions: 1:30-5:00p (except Monday afternoon ends at 6:15). Lectures are maximally 45-60 min with 15 min break.

Course Directors:
Sean Zhang               Johns Hopkins Hospital/Johns Hopkins School of Medicine, USA
Sybren de Hoog        Center of Expertise in Mycology of RadboudUMC, Nijmegen, The Netherlands
Paschalis Vergidis     Mayo Clinic, USA
Nancy Wengenack    Mayo Clinic, USA

Course Faculty:
Barbara Alexander    Duke University Medical Center
Esther Babady          Memorial Sloan Kettering Cancer Center
Avrom Caplan           New York University Grossman School of Medicine
Kimberly Hanson       ARUP
Shawn Lockhart        CDC Mycotic Diseases Branch
Hong Nguyen            University of Pittsburgh Medical Center
Thomas Walsh          Center for Innovative Therapeutics and Diagnostics
Nathan Wiederhold    Fungus Testing Laboratory, University of Texas San Antonio     
Elitza Theel                Mayo Clinic
Andrew Norgan         Mayo Clinic
Gary Procop              American Board of Pathology
Roxana G. Vitale       CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas), Hospital JM Ramos Mejía, Buenos Aires, Argentina

Technical and Administrative Assistance:
Susan Reigel            Administrative Assistance, Mayo Clinic
Melissa Frechette      Teaching Assistance, Mayo Clinic
Amber Milone           Teaching Assistance, Mayo Clinic
Warda Memon          Teaching Assistance, Johns Hopkins Hospital
Rachel Green           Teaching Assistance, Johns Hopkins Hospital  
Xin Zhou                        Teaching Assistance, Radbound University

Venue:
Mayo Clinic, Rochester, Minnesota, U.S.A.

Costs and requirements:
The course fee for 6 full days course is US$ 1,000, including breakfast, luncheons, coffee/tea with snacks/cookies during breaks. The course materials are based on the Atlas of Clinical Fungi (by Sybren de Hoog, Josep Guarro, …, and Roxana Vitale, which can be purchased at https://www.atlasclinicalfungi.org/ and includes an electronic searchable version). Please bring your laptop and a lab-coat. A maximum of 60 participants is allowed

Provisional program:

Monday (9/23):      The fungal Kingdom; Introduction
7:15 am Breakfast
Lectures: 8:00-9:35a and 9:45a-12:45p (4.5 hours)

• 8:00-8:05: Course Directors: Welcome & Introduction. 
• 8:05-9:05: Sybren de Hoog: General taxonomy; life cycles; pleomorphism, nomenclature
• 9:05-9:35: Sybren de Hoog: Fungal diseases of animals & Zoonoses
• 9:35-9:45: Break
• 9:45-10:45: Sybren de Hoog: Introduction to Atlas of Clinical Fungi; diagnostic keys
• 10:45-11:45: Sybren de Hoog: Taxonomy and solutions for nomenclatural stability.
• 11:45-12:45: Thomas Walsh: The host, who are at risk for fungal diseases?

Lunch: 12:45-1:45p 

Lectures: 1:45-3:15p and 3:30-5:30p (3.5 hours)        

• 1:45-2:15: Nancy Wengenack: Principles and procedures for detection and culture of fungi in clinical specimens
• 2:15-2:45: Sybren de Hoog: Introduction to microscopic methods.
• 2:45-3:15: Sean Zhang: Direct microscopic examination on clinical specimens
• 3:15-3:30: Break
• 3:30-4:30: Nathan Wiederhold: Practice of antifungal testing, PK/PD of antifungals, antifungal Therapeutic Drug Monitoring
• 4:30-5:30: Shawn Lockhart: Antifungal drug acquired and intrinsic resistance.

Tuesday (9/24):      Mucorales and Entomophthorales & Eurotiales
7:15 am Breakfast
Lectures: 8:00-10:00a and 10:15a-12:45p (4.5 hours)

• 8:00-9:00: Sybren de Hoog: Taxonomy of the Mucorales and Entomophthorales.
• 9:00-10:00: Thomas Walsh: Infections by Mucorales and Entomophthorales.
• 10:00-10:15: Break
• 10:15-10:30: Sybren de Hoog: Short introduction to ascomycetes.
• 10:30-11:00: Sybren de Hoog: Conidiogenesis in asexual fungi: hyphomycetes and coelomycetes.
• 11:00-11:45: Sybren de Hoog: Taxonomy of Eurotiales: Aspergillus, Paecilomyces, Talaromyces.
• 11:45-12:45: Barbara Alexander: Aspergillosis.

Lunch: 12:45-1:45p (Non-CME Product Theater: Pearl Diagnostics 1:00 - 1:15 | Non-CME Product Theater: T2 Biosystems 1:25 - 1:40)

Practice: 1:45-3:30pm and 3:45-5:15pm (3.25 hours)

• 1:45-3:30: Microscopy mucormycetes (Lichtheimia ramosa, Cunninghamella bertholletiae, Mucor circinelloides, Rhizopus arrhizus, Syncephalastrum racemosum, Rhizomucor pusillus).
• 3:30-3:45: Break
• 3:45-5:15: Microscopy of Eurotiales (Aspergillus fumigatus, Aspergillus terreus, Aspergillus calidoustus, Paecilomyces variotii, Rasamsonia argillacea, Talaromyces [Penicillium] purpureogenus).

Wednesday (9/25):                       Onygenales
7:45 am Breakfast
Lectures: 8:30-9:45a and 10:00a-12:45p (4 hours)

• 8:30-9:00: Sybren de Hoog: Overview of the order Onygenales.
• 9:00-9:45: Sybren de Hoog: Arthrodermataceae: dermatophytes.
• 9:45-10:00: Break
• 10:00-11:00: Avrom Caplan: Dermatophyte infections, a Dermatologist's Perspective.
• 11:00-11:45: Sybren de Hoog: Ajellomycetaceae: systemic mycoses.
• 11:45-12:45: Paschalis Vergidis: Coccidioidomycosis, Paracoccidioidomycosis, Blastomycosis, Histoplasmosis, Emergomycosis, Talaromycosis.

Lunch: 12:45-1:45p (Non-CME Product Theater: Bruker 1:00 - 1:15 | Non-CME Product Theater: Melinta Therapeutics 1:25 - 1:40)

Practice: 1:45-3:30pm and 3:45-5:15pm (3.25 hours)

• 1:45-3:30: Microscopy Dermatophytes (Epidermophyton floccosum, Microsporum canis, Nannizzia gypsea [Microsporum gypserum], Trichophyton interdigitale, Trichophyton rubrum, Trichophyton tonsurans, Trichophyton [Arthroderma] benhamiae).
• 3:30-3:45: Break
• 3:45-4:45: Microscopy review of endemic mycoses (Histoplasma capsulatum, Coccidioides immitis/posadasii, Blastomyces dermatitidis).
• 4:45-5:15: Andrew Norgan: Histopathology review of endemic mycoses (Histoplasmosis, Coccidioidomycosis, Blastomycosis, Paracoccidioidomycosis, Talaromycosis, Emergomycosis)

Thursday (9/26):    Yeasts and Yeast-like fungi
7:45 am Breakfast
Lectures: 8:30-10:30a and 10:45a-12:45p (4 hours)

• 8:30-9:30: Sybren de Hoog: Overview of yeasts and yeast-like fungi.
• 9:30-10:30: Hong Nguyen: Candidiasis
• 10:30-10:45: Break
• 10:45-11:45: Kimberly Hanson: Cryptococcosis
• 11:45-12:45: Esther Babady: Pneumocystis

Lunch: 12:45-1:45p (Non-CME Product Theater: CHROMagar 1:00 - 1:15)

Practice: 1:45-3:30pm and 3:45-5:15pm (3.25 hours)

• 1:45-2:45: Microscopy of ascomycetous yeasts (Candida albicans, Candida glabrata [Nakaseomyces glabrataa], Candida krusei [Pichia kudriavzevii], Candida tropicalis, Magnusiomyces capitatus [Saprochaete capitata], Geotrichum candidum
• 2:45-3:30: Microscopy of basidiomycetous yeasts (Cryptococcus neoformans, Malassezia furfur, Trichosporon asahii, Rhodotorula mucilaginosa).
• 3:30-3:45: Break
• 3:45-4:30: Elitza Theel: Fungal serology
• 4:30-5:15: Sean Zhang: Current diagnostic techniques – MALDI-TOF MS, fungal biomarkers, molecular and sequencing identification

Friday (9/27):         Black yeasts and other dematiaceous opportunists
7:45 am Breakfast
Lectures: 8:30-10:30a and 10:45a-12:45p (4 hours)

• 8:30-9:30: Sybren de Hoog: Taxonomy of black yeasts and relatives; diagnostic overview.
• 9:30-10:30: Sybren de Hoog: Clinical pictures of black yeasts and relatives.
• 10:30-10:45: Break
• 10:45-11:45: Roxana Vitale: Chromoblastomycosis & Emerging and common mycoses in South America
• 11:45-12:45: Sybren de Hoog: Mycetoma, melanized opportunists in Pleosporales (dematiaceae, coelomycetes).

Lunch: 12:45-1:45p (Non-CME Product Theater: DiaSorin Molecular LLC 1:00 - 1:15)

Practice: 1:45-3:30pm and 3:45-5:15pm (3.25 hours)

• 1:45-3:30: Microscopy of traumatic Chaetothyriales (Exophiala dermatitidis, Cladophialophora emmonsii, Phialophora verrucosa, Rhinocladiella similis).
• 3:30-3:45: Break
• 3:45-5:15: Microscopy of Pleosporales and other melanised fungi (Alternaria alternata, Curvularia geniculata, Bipolaris spicifera, Phaeoacremonium parasiticum, Phoma herbarum).

Saturday (9/28):    Hyaline opportunists and conclusion
7:45 am Breakfast
Lectures: 8:30-10:30a and 10:45a-12:45p (4 hours)

• 8:30-9:00: Sybren de Hoog: Ophiostomatales: Sporothrix and sporotrichosis.
• 9:00-10:00: Sybren de Hoog: Hypocreales: Fusarium and relatives.
• 10:00-10:30: Sybren de Hoog: Microascales: Scedosporium and brain infection.
• 10:30-10:45: Break
• 10:45-11:45: Sybren de Hoog: Basidiomycetous filamentous fungi.
• 11:45-12:45: Gary Procop: Fungal histopathology.

Lunch: 12:45-1:45p

Practice: 1:45-3:15p and 3:30-5:00 (3 hours)

• Microscopy of filamentous Basidiomycetes (Schizophyllum commune, Phanerochaete chrysosporium [Sporotrichum pruinosum]).
• Microscopy of Hypocreales (Fusarium oxysporum, Sarocladium [Acremonium] kiliense).
• Microscopy of Microascales (Lomentospora [Scedosporium] prolificans), Scedosporium apiospermum, Microascus paisii [Scopulariopsis brumptii]).
• Microscopy of Ophiostomatales (Sporothrix schenckii).
• 3:15-3:30: Break
• 3:30-5:00: Sean Zhang & Paschalis Vergidis: Case studies

Certificates and Farewell 5:00-5:15pm